| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| R Westermann, M Johannsen, K Unsicker, C Groth. Basic fibroblast growth factor (bFGF) immunoreactivity is present in chromaffin granules. Journal of neurochemistry. vol 55. issue 1. 1990-07-20. PMID:2192016. |
western blot analysis revealed the presence of the typical 18-kda bfgf and additional immunoreactive materials with molecular masses of approximately 24, 30, and 46 kda in whole bovine adrenal, and in cortex and medulla. |
1990-07-20 |
2023-08-11 |
chicken |
| Y C Lee, W J Driscoll, C A Strot. Charge isoforms of the adrenocortical pregnenolone-binding protein: influence of phosphorylation on isoformation and binding activity. Proceedings of the National Academy of Sciences of the United States of America. vol 87. issue 5. 1990-04-06. PMID:2308958. |
western blot analysis revealed that the pbp was present in both the fasciculata and reticularis of the adrenal cortex, though the isoform patterns were not identical for the two zones. |
1990-04-06 |
2023-08-11 |
Not clear |
| J Srinivasan, A P Hays, F P Thomas, S A Sadiq, K H Barth, R Liem, M A Mena, J G DeYebenes, N Lato. Autoantigens in human neuroblastoma cells. Journal of neuroimmunology. vol 26. issue 1. 1990-02-08. PMID:1688443. |
northern blot analysis revealed a single rna band of 4800 bp for nfh in normal brain but two rna species of 4800 and 3800 bp in both neuroblastoma and adrenal cells, confirming their common origin. |
1990-02-08 |
2023-08-11 |
human |
| D C Wan, C L Choi, B G Livet. Developmental expression of proenkephalin A mRNA and phenylethanolamine N-methyltransferase mRNA in foetal sheep adrenal medulla. International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience. vol 7. issue 5. 1989-12-21. PMID:2816486. |
northern blot analysis of rna extracts from foetal adrenals demonstrated that proenk a mrna was expressed as early as 60 days of gestation, a time at which the foetal adrenal is not functionally innervated. |
1989-12-21 |
2023-08-11 |
Not clear |
| R K Sharma, R B Marala, T M Dud. Purification and characterization of the 180-kDa membrane guanylate cyclase containing atrial natriuretic factor receptor from rat adrenal gland and its regulation by protein kinase C. Steroids. vol 53. issue 3-5. 1989-11-21. PMID:2572076. |
in rat adrenal glands we now demonstrate: 1) the direct presence of a 180-kda anf-binding protein in gtp-affinity purified membrane fraction as evidenced by affinity cross-linking technique and by the western blot analysis of the partially purified enzyme; 2) that the enzyme is biochemically and immunologically different from the soluble guanylate cyclase as there is no antigenic cross-reactivity of 180-kda guanylate cyclase antibody with soluble guanylate cyclase; 3) in contrast to the soluble guanylate cyclase, the particulate enzyme is not stimulated by nitrite-generating compounds and hemin; and 4) protein kinase c inhibits both the basal and anf-dependent guanylate cyclase activity and phosphorylates the 180-kda guanylate cyclase. |
1989-11-21 |
2023-08-11 |
mouse |
| R J Parmer, A H Koop, M T Handa, D T O'Conno. Molecular cloning of chromogranin A from rat pheochromocytoma cells. Hypertension (Dallas, Tex. : 1979). vol 14. issue 4. 1989-11-08. PMID:2793216. |
northern blot experiments demonstrated the presence of an approximately 2.2 kb rat cga mrna in a neuroendocrine distribution (adrenal, brain, pheochromocytoma cells, but not skeletal muscle, heart, or kidney). |
1989-11-08 |
2023-08-11 |
human |
| F P Chou, S Gallant, A C Browni. Cytochromes P-45011 beta, P-450scc and adrenodoxin gene expression during adrenocortical regeneration in the rat. The Journal of endocrinology. vol 122. issue 1. 1989-10-03. PMID:2788699. |
plasmids containing cdna inserts coding for cytochrome p-45011 beta, cytochrome p-450scc and adrenodoxin were used to determine, by northern blot analysis, mrna levels at 1, 2 and 3 weeks of adrenal regeneration. |
1989-10-03 |
2023-08-11 |
rat |
| D C Wan, D Scanlon, C L Choi, S J Bunn, P R Howe, B G Livet. Co-localization of RNAs coding for phenylethanolamine N-methyltransferase and proenkephalin A in bovine and ovine adrenals. Journal of the autonomic nervous system. vol 26. issue 3. 1989-08-31. PMID:2754179. |
characterization of this probe by northern blot hybridization showed that it hybridized to a single band in rna extracted from bovine and ovine adrenal medullae. |
1989-08-31 |
2023-08-11 |
cattle |
| G M Pasinetti, D G Morgan, S A Johnson, S P Lerner, M A Myers, J Poirier, C E Finc. Combined in situ hybridization and immunocytochemistry in the assay of pharmacological effects on tyrosine hydroxylase mRNA concentration. Pharmacological research. vol 21. issue 3. 1989-08-17. PMID:2568627. |
finally, the reserpine-induced increase in adrenal th mrna was used to validate quantitative aspects of the in situ hybridization technique by comparison with blot hybridization. |
1989-08-17 |
2023-08-11 |
Not clear |
| N Rost, M Chaffanet, M F Nissou, C Chauvin, A M Foote, M Lainé, A L Benabi. Expression of the preproenkephalin A gene in tumor cells and brain glioma: a northern and in situ hybridization study. Neuropeptides. vol 13. issue 2. 1989-08-10. PMID:2739883. |
preproenkephalin a (ppa) mrna expression was studied by northern blot and in situ hybridization in cell lines (rat glioma c6, rat hepatoma htc, human neuroblastoma imr32, mouse neuroblastoma ns20y, rat fibroblast fr3t3, human bladder carcinoma ej, human vulva carcinoma a431, myelocytic leukemia hl60, rat adrenal carcinoma y1) and in brain tumours (implanted c6 cells). |
1989-08-10 |
2023-08-11 |
mouse |
| D J Campbell, J F Habene. Hybridization in situ studies of angiotensinogen gene expression in rat adrenal and lung. Endocrinology. vol 124. issue 1. 1989-02-07. PMID:2909366. |
both rat adrenal and lung contain low levels of angiotensinogen mrna, as shown by northern blot and nuclease s1 analyses of rna extracted from these tissues. |
1989-02-07 |
2023-08-11 |
rat |
| E I Ginns, M Rehavi, B M Martin, M Weller, K L O'Malley, M E LaMarca, C G McAllister, S M Pau. Expression of human tyrosine hydroxylase cDNA in invertebrate cells using a baculovirus vector. The Journal of biological chemistry. vol 263. issue 15. 1988-06-20. PMID:2896667. |
western blot analysis of extracts of sf9 cells infected with the recombinant baculovirus containing human tyrosine hydroxylase cdna revealed a major immunoreactive band with an apparent mr of 60 kda, identical to the size of the immunoreactive protein from rat adrenal and caudate nucleus. |
1988-06-20 |
2023-08-11 |
human |
| K M McMasters, L A Dickson, R V Shamy, K Robischon, G J Macdonald, W R Moyl. Rat cholesterol side-chain cleavage enzyme (P-450scc): use of a cDNA probe to study the hormonal regulation of P-450scc mRNA levels in ovarian granulosa cells. Gene. vol 57. issue 1. 1988-03-07. PMID:3123325. |
northern blot analysis indicates that p-450scc mrna is present in steroidogenic tissues (ovary, adrenal, testis), but not in brain, kidney, liver, lung, or heart. |
1988-03-07 |
2023-08-11 |
human |
| M H Doolittle, H Wong, R C Davis, M C Schot. Synthesis of hepatic lipase in liver and extrahepatic tissues. Journal of lipid research. vol 28. issue 11. 1988-03-02. PMID:3323389. |
immunoblot analysis of partially purified adrenal lipase showed an immunoreactive band co-migrating with hl-ii at 53,000 daltons which was absent in a control blot treated with preimmune serum. |
1988-03-02 |
2023-08-11 |
rat |
| I Hanukoglu, T Gutfinger, M Haniu, J E Shivel. Isolation of a cDNA for adrenodoxin reductase (ferredoxin-NADP+ reductase). Implications for mitochondrial cytochrome P-450 systems. European journal of biochemistry. vol 169. issue 3. 1988-02-18. PMID:3691502. |
rna blot analysis indicates that in the adrenal cortex and corpus luteum there is only one major mrna (approximately 2000 bp) for ar. |
1988-02-18 |
2023-08-11 |
human |
| K L Fisher, C M Gorman, G A Vehar, D P O'Brien, R M Law. Cloning and expression of human tissue factor cDNA. Thrombosis research. vol 48. issue 1. 1988-02-02. PMID:3424286. |
tissue factor mrna of 2400 nucleotides was detected in adipose, adrenal, small intestine and a number of other tissues by northern blot hybridization analysis. |
1988-02-02 |
2023-08-11 |
human |
| T Lacaze-Masmonteil, Y de Keyzer, J P Luton, A Kahn, X Bertagn. Characterization of proopiomelanocortin transcripts in human nonpituitary tissues. Proceedings of the National Academy of Sciences of the United States of America. vol 84. issue 20. 1987-11-25. PMID:3478693. |
blot hybridization analysis of normal adrenal, thymus, and testis rnas revealed a small rna species approximately 400 nucleotides shorter than the 1200-nucleotide pituitary species. |
1987-11-25 |
2023-08-11 |
human |
| H E Shubeita, J F Sambrook, A M McCormic. Molecular cloning and analysis of functional cDNA and genomic clones encoding bovine cellular retinoic acid-binding protein. Proceedings of the National Academy of Sciences of the United States of America. vol 84. issue 16. 1987-09-18. PMID:3039499. |
rna blot hybridization analysis using pcrabp-hs1 as a probe revealed a single 1050-nucleotide mrna species in bovine adrenal, uterus, and testis, tissues that contain the highest levels of retinoic acid-binding activity. |
1987-09-18 |
2023-08-11 |
cattle |
| R F Chanderbhan, A T Kharroubi, B J Noland, T J Scallen, G V Vahoun. Sterol carrier protein2: further evidence for its role in adrenal steroidogenesis. Endocrine research. vol 12. issue 4. 1987-04-27. PMID:3030719. |
western blot analysis of adrenal subcellular fractions indicates the presence of a protein of identical molecular weight and at least similar antigenicity as homogeneous rat liver scp2. |
1987-04-27 |
2023-08-11 |
rat |
| S A Limborska, S A Korneev, N E Maleeva, P A Slominsky, A G Jincharadze, P L Ivanov, A P Rysko. Cloning of Alu-containing cDNAs from human fibroblasts and identification of small Alu+ poly(A)+ RNAs in a variety of human normal and tumor cells. FEBS letters. vol 212. issue 2. 1987-04-02. PMID:2434358. |
dna of the clones was used for northern blot hybridization analysis of a number of poly(a)-containing rnas from normal human tissues (brain, stomach, uterus, spleen, fibroblasts) and tumors (neurinoma, glioma, neuroblastoma, liposarcoma, adrenal cortex adenocarcinoma). |
1987-04-02 |
2023-08-11 |
human |