| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Shwu-Fen Chang, Hsien-Hsiung Le. Analysis of the CYP21A2 gene with intergenic recombination and multiple gene deletions in the RCCX module. Genetic testing and molecular biomarkers. vol 15. issue 1-2. 2011-07-19. PMID:21117955. |
to determine rccx alterations, we used the polymerase chain reaction (pcr) product containing the tenascin b (tnxb) and cyp21a2 genes with taqi digestion and southern blot analysis with asei and ndei endonuclease digestion of genomic dna from congenital adrenal hyperplasia patients with common mutations resulting from an intergenic conversion of cyp21a1p, such as an i2 splice, i172n, v281l, f306-l307inst, q318x, and r356w, and dual mutations of i236n/v237e in the cyp21a2 gene. |
2011-07-19 |
2023-08-12 |
Not clear |
| Hsien-Hsiung Lee, Yann-Jinn Lee, Mei-Chyn Cha. Comparing the Southern blot method and polymerase chain reaction product analysis for chimeric RCCX detection in CYP21A2 deficiency. Analytical biochemistry. vol 399. issue 2. 2010-05-20. PMID:19961824. |
we used the polymerase chain reaction (pcr) product and southern blot method with taqi endonuclease digestion to identify a chimeric rccx module in two unrelated patients with congenital adrenal hyperplasia (cah). |
2010-05-20 |
2023-08-12 |
Not clear |
| Charlotte Dubé, Francis Bergeron, Marie-Josée Vaillant, Nicholas M Robert, Catherine Brousseau, Jacques J Trembla. The nuclear receptors SF1 and LRH1 are expressed in endometrial cancer cells and regulate steroidogenic gene transcription by cooperating with AP-1 factors. Cancer letters. vol 275. issue 1. 2009-02-12. PMID:19022561. |
here we used rt-pcr and western blot to show that the nuclear receptors sf1 and lrh1, two well-known regulators of steroidogenic gene expression in gonadal and adrenal cells, are also expressed in endometrial cancer cell lines. |
2009-02-12 |
2023-08-12 |
human |
| Amita Kapoor, Jason Leen, Stephen G Matthew. Molecular regulation of the hypothalamic-pituitary-adrenal axis in adult male guinea pigs after prenatal stress at different stages of gestation. The Journal of physiology. vol 586. issue 17. 2008-12-18. PMID:18635650. |
corticosteroid receptors in the hippocampus, hypothalamus and pituitary were measured, as well as corticotropin-releasing hormone (crh), pro-opiomelanocortin (pomc) and adrenal enzymes in the paraventricular nucleus, pituitary and adrenal cortex, respectively, by in situ hybridization and western blot. |
2008-12-18 |
2023-08-12 |
human |
| Parveen Abidi, Susan Leers-Sucheta, Yuan Cortez, Jiahuai Han, Salman Azha. Evidence that age-related changes in p38 MAP kinase contribute to the decreased steroid production by the adrenocortical cells from old rats. Aging cell. vol 7. issue 2. 2008-03-28. PMID:18241324. |
western blot analysis indicated that aging caused increased phosphorylation and activation of rat adrenal p38 mapk, but not the erk1/2 or jnk1/2. |
2008-03-28 |
2023-08-12 |
rat |
| Salvatore Valiante, Marina Prisco, Rosaria Sciarrillo, Maria De Falco, Anna Capaldo, Flaminia Gay, Piero Andreuccetti, Vincenza Laforgia, Lorenzo Varan. Pituitary adenylate cyclase-activating polypeptide, vasoactive intestinal polypeptide and their receptors: distribution and involvement in the secretion of Podarcis sicula adrenal gland. The Journal of endocrinology. vol 196. issue 2. 2008-03-11. PMID:18252952. |
using 'far western blot' technique, we showed the presence of specific binding sites for vip/pacap in the adrenal glands of the lizard. |
2008-03-11 |
2023-08-12 |
Not clear |
| Raed A El-Hajj, Susan B McKay, Dennis B McKa. Pharmacological and immunological identification of native alpha7 nicotinic receptors: evidence for homomeric and heteromeric alpha7 receptors. Life sciences. vol 81. issue 16. 2007-11-26. PMID:17928008. |
when adrenal nachrs are immunoprecipated with mab319, an antibody which recognizes alpha7 nachr protein, and then probed with mab319 using western blot analysis, a single band of approximately 53 kda is identified. |
2007-11-26 |
2023-08-12 |
cattle |
| Raed A El-Hajj, Susan B McKay, Dennis B McKa. Pharmacological and immunological identification of native alpha7 nicotinic receptors: evidence for homomeric and heteromeric alpha7 receptors. Life sciences. vol 81. issue 16. 2007-11-26. PMID:17928008. |
when adrenal nachrs are immunoprecipated with mab35, an antibody which recognizes alpha3 and alpha5 nachr proteins, and then probed with mab319 using western blot analysis, a single band of approximately 53 kda is identified. |
2007-11-26 |
2023-08-12 |
cattle |
| Q Li, X-Q Zhang, L Nie, G-S Chen, H Li, F Zhang, L-Y Zhang, L Hong, S-F Wang, H Wan. Expression of interferon-gamma in human adrenal gland and kidney tumours. British journal of cancer. vol 97. issue 3. 2007-10-02. PMID:17622250. |
western blot analysis has further confirmed the immunohistochemistry results by showing a distinct ifn-gamma band corresponding to 17.4 kda in tissue extracts from adrenal cortical adenoma, phaeochromocytoma and clear cell rccs. |
2007-10-02 |
2023-08-12 |
human |
| Shintaro Maru, Noboru Yamashita, Yuichiro Shinn. [Adrenal multilocular echinococcosis: a case report]. Nihon Hinyokika Gakkai zasshi. The japanese journal of urology. vol 98. issue 4. 2007-07-13. PMID:17564109. |
by the pathological findings and the serological tests (elisa, and western blot examination), the tumor was diagnosed as an adrenal multilocular echinococcosis. |
2007-07-13 |
2023-08-12 |
Not clear |
| Alina Nico West, Geoffrey A Neale, Stanley Pounds, Bonald C Figueredo, Carlos Rodriguez Galindo, Mara Albonei D Pianovski, Antonio G Oliveira Filho, David Malkin, Enzo Lalli, Raul Ribeiro, Gerard P Zambett. Gene expression profiling of childhood adrenocortical tumors. Cancer research. vol 67. issue 2. 2007-02-20. PMID:17234769. |
distinct patterns of gene expression, validated by quantitative real-time pcr and western blot analysis, were identified that distinguish normal adrenal cortex from tumor. |
2007-02-20 |
2023-08-12 |
Not clear |
| Feng-Jie Lai, Ya-Chieh Hsin, Shiue-Chin Huang, Ching-Li Cheng, Shih-Chie Hsin, Ming-Chia Hsieh, Shyi-Jang Shi. Down-regulation of adrenal neuronal nitric oxide synthase mRNAs and proteins after deoxycorticosterone acetate-salt treatment in rats. The Journal of steroid biochemistry and molecular biology. vol 101. issue 4-5. 2007-01-16. PMID:16965914. |
adrenal nnos was also detected by western blot in five vehicle-treated and five doca-salt-treated rats. |
2007-01-16 |
2023-08-12 |
rat |
| Yuko Katoh-Fukui, Akiko Owaki, Yoshiro Toyama, Masatomo Kusaka, Yuko Shinohara, Mamiko Maekawa, Kiyotaka Toshimori, Ken-Ichirou Morohash. Mouse Polycomb M33 is required for splenic vascular and adrenal gland formation through regulating Ad4BP/SF1 expression. Blood. vol 106. issue 5. 2005-09-29. PMID:15899914. |
western blot, immunohistochemistry, and reverse transcriptase-polymerase chain reaction (rt-pcr) analyses of the m33 knock-outs all indicated significantly low expression of adrenal 4 binding protein/steroidogenic factor-1 (ad4bp/sf-1), indicating that m33 is an essential upstream regulator of ad4bp/sf1. |
2005-09-29 |
2023-08-12 |
mouse |
| Shin-Ichi Izumi, Kuniko Abe, Tomayoshi Hayashi, Paul K Nakane, Takehiko Koj. Immunohistochemical localization of the ACTH (MC-2) receptor in the rat placenta and adrenal gland. Archives of histology and cytology. vol 67. issue 5. 2005-06-14. PMID:15781985. |
antibodies against synthetic acthr peptides were raised in rabbits, and western blot analysis showed that the antibody reacted with specific proteins in the rat adrenal glands and placentas. |
2005-06-14 |
2023-08-12 |
rat |
| Marita K Broadhurst, Rita S-F Lee, Sarah Hawkins, Thomas T Wheele. The p100 EBNA-2 coactivator: a highly conserved protein found in a range of exocrine and endocrine cells and tissues in cattle. Biochimica et biophysica acta. vol 1681. issue 2-3. 2005-03-09. PMID:15627504. |
western blot and immunohistochemical analyses revealed that the bovine p100 protein is present in a range of exocrine and endocrine cells and tissues, including the lactating mammary gland, pancreas, adrenal, parotid, anterior pituitary, corpus luteum, ovarian follicular cells, placenta and small intestine. |
2005-03-09 |
2023-08-12 |
human |
| Judith A Enyeart, Sanjay J Danthi, John J Enyear. TREK-1 K+ channels couple angiotensin II receptors to membrane depolarization and aldosterone secretion in bovine adrenal glomerulosa cells. American journal of physiology. Endocrinology and metabolism. vol 287. issue 6. 2004-12-16. PMID:15315905. |
northern blot and in situ hybridization studies demonstrated that btrek-1 mrna is uniformly distributed in the bovine adrenal cortex, including zona fasciculata and zona glomerulosa, but is absent from the medulla. |
2004-12-16 |
2023-08-12 |
rat |
| Hsien-Hsiung Lee, Yann-Jinn Lee, Ching-Yu Li. PCR-based detection of the CYP21 deletion and TNXA/TNXB hybrid in the RCCX module. Genomics. vol 83. issue 5. 2004-10-26. PMID:15081125. |
detection of the cyp21 deletion in congenital adrenal hyperplasia (cah) in the rccx module has been previously done by southern blot analysis with multiple probes and separate digestions with the restriction endonucleases taqi and bglii, which is laborious and indirect. |
2004-10-26 |
2023-08-12 |
Not clear |
| Moshe Reichenstein, Reuven Reich, Jean-Guy LeHoux, Israel Hanukogl. ACTH induces TIMP-1 expression and inhibits collagenase in adrenal cortex cells. Molecular and cellular endocrinology. vol 215. issue 1-2. 2004-10-21. PMID:15026182. |
northern blot analysis of total rna from rat adrenal zona fasciculata-reticularis and zona glomerulosa showed that in both of these zones timp-1 expression was induced within 12h after acth injection. |
2004-10-21 |
2023-08-12 |
rat |
| Hsien-Hsiung Lee, Yann-Jinn Lee, Peter Chan, Ching-Yu Li. Use of PCR-based amplification analysis as a substitute for the southern blot method for CYP21 deletion detection in congenital adrenal hyperplasia. Clinical chemistry. vol 50. issue 6. 2004-07-14. PMID:15161727. |
use of pcr-based amplification analysis as a substitute for the southern blot method for cyp21 deletion detection in congenital adrenal hyperplasia. |
2004-07-14 |
2023-08-12 |
Not clear |
| Asish K Ghosh, Robert Steele, Ratna B Ra. Modulation of human luteinizing hormone beta gene transcription by MIP-2A. The Journal of biological chemistry. vol 278. issue 26. 2003-08-20. PMID:12700240. |
rna dot blot analysis of human multiple tissue expression array suggested that mip-2a is highly abundant in right cerebellum, pituitary, adrenal, and testis but barely detectable in skeletal muscle. |
2003-08-20 |
2023-08-12 |
human |